The following are the blood tests for specific markers and details of process:
HAEMATOLOGY
HAEMOGRAM
Total WBC Count
- Method: WB/Automated
- Specimen: EDTA WHOLE BLOOD
- Unit of measure: cells/cumm
- Biological reference interval: 4000-10000
DIFFERENTIAL COUNT(DC)
Neutrophils
- Method: Optical(light scatter)Microscopy
- Specimen: EDTA WHOLE BLOOD
- Unit of measure: %
- Biological reference interval: 40-80
Lymphocytes
- Method: Optical(light scatter)Microscopy
- Specimen: EDTA WHOLE BLOOD
- Unit of measure: %
- Biological reference interval: 20-40
Monocytes
- Method: Optical(light scatter)Microscopy
- Specimen: EDTA WHOLE BLOOD
- Unit of measure: %
- Biological reference interval: 2-8
Eosinophils
- Method: Optical(light scatter)Microscopy
- Specimen: EDTA WHOLE BLOOD
- Unit of measure: %
- Biological reference interval: 1-6
Basophils
- Method: Optical(light scatter)Microscopy
- Specimen: EDTA WHOLE BLOOD
- Unit of measure: %
- Biological reference interval: 0-1
IMMATURE GRANULOCYTE PERCENTAGE(IG%)
- Method : Fluorescent Flow Cytometry-SF Cube
- Specimen: EDTA WHOLE BLOOD
- Unit of measure: %
- Biological reference interval: —
RBC (Red Blood Cell Count)
- Method: WB/Automated
- Specimen: EDTA WHOLE BLOOD
- Unit of measure: Million/cmm
- Biological reference interval:
Male: 4.5-5.5
Female: 3.8-4.8
Haemoglobin (HB)
- Method: WB/Automated
- Specimen: EDTA WHOLE BLOOD
- Unit of measure: g/dl
- Biological reference interval:
Male: 13.0-17.0
Female: 12.0-15.0
PCV -( Haematocrit-Packed Cell Volume)
- Method: WB/Automated
- Specimen: EDTA WHOLE BLOOD
- Unit of measure: %
- Biological reference interval:
Male: 40-50
Female: 36-46
MCV (Mean Corpuscular Volume)
- Method: WB/Automated
- Specimen: EDTA WHOLE BLOOD
- Unit of measure: fl
- Biological reference interval: 83-101
MCH (Mean Corpuscular Hemoglobin )
- Method: Calculated
- Specimen: EDTA WHOLE BLOOD
- Unit of measure: pg
- Biological reference interval: 27-31
MCHC (Mean Corpuscular Hemoglobin Concentration)
- Method: WB/Automated
- Specimen: EDTA WHOLE BLOOD
- Unit of measure: %
- Biological reference interval: 31.5-34.5
Platelet Count
- Method: WB/Automated
- Specimen: EDTA WHOLE BLOOD
- Unit of measure: Lakhs/cumm
- Biological reference interval: 1.5-4.5
RDW-SD(Red Cell Distribution Width-SD)
- Method: Derived from RBC Histogram
- Specimen: EDTA WHOLE BLOOD
- Unit of measure: fl
- Biological reference interval: 39-46
RDW-CV(Red Cell Distribution Width-CV)
- Method: Derived from RBC Histogram
- Specimen: EDTA WHOLE BLOOD
- Unit of measure: %
- Biological reference interval: 11.6-14.0
PDW(Platelet Distribution Width)
- Method: Derived from platelet Histogram
- Specimen: EDTA WHOLE BLOOD
- Unit of measure: %
- Biological reference interval: 8.3-56.6
MPV (Mean Platelet Volume)
- Method: Derived from platelet Histogram
- Specimen: EDTA WHOLE BLOOD
- Unit of measure: fl
- Biological reference interval: —
PLCR(Platelet to Large Cell Ratio)
- Method: —
- Specimen: EDTA WHOLE BLOOD
- Unit of measure: %
- Biological reference interval: —
PCT(Plateletcrit)
- Method: Calculated
- Specimen: EDTA WHOLE BLOOD
- Unit of measure: %
- Biological reference interval: —
Absolute Neutrophil Count
- Method: Fluorescent Flow Cytometry-SF Cube
- Specimen: EDTA WHOLE BLOOD
- Unit of measure: cells/cumm
- Biological reference interval: 2000-7000
Absolute Lymphocyte Count
- Method: Fluorescent Flow Cytometry-SF Cube
- Specimen: EDTA WHOLE BLOOD
- Unit of measure: cells/cumm
- Biological reference interval: 1000-3000
Absolute Eosinophil Count
- Method: Fluorescent Flow Cytometry-SF Cube
- Specimen: EDTA WHOLE BLOOD
- Unit of measure: cells/cumm
- Biological reference interval: 20-500
Absolute Monocytes Count
- Method: Fluorescent Flow Cytometry-SF Cube
- Specimen: EDTA WHOLE BLOOD
- Unit of measure: cells/cumm
- Biological reference interval: 200-1000
Absolute Basophils Count
- Method: Fluorescent Flow Cytometry-SF Cube
- Specimen: EDTA WHOLE BLOOD
- Unit of measure: cells/cumm
- Biological reference interval: 20-100
Neutrophil to lymphocyte Ratio(NLR)
- Method: —
- Specimen: EDTA WHOLE BLOOD
- Unit of measure: —
- Biological reference interval: —
RETICULOCYTE COUNT
- Method: —
- Specimen: EDTA WHOLE BLOOD
- Unit of measure: %
- Biological reference interval: 0.5-2.5
Erythrocyte Sedimentation Rate(ESR)
1 Hour
- Method: Westergran Method
- Specimen: EDTA WHOLE BLOOD
- Unit of measure: mm/hr
- Biological reference interval:
Male: <10
Female: <12.0
FIBRINOGEN
- Method: —
- Specimen: CITRATE PLASMA
- Unit of measure: mg/dL
- Biological reference interval: 220-500
BIOCHEMISTRY
GLUCOSE FASTING (FBS)
- Method: Glucose Oxidase – Peroxidase
- Specimen: FLUORIDE PLASMA
- Unit of measure: mg/dL
- Biological reference interval:
Normal:74-100
Pre Diabetes:100-125
Diabetes :>=126
ADA(American Diabetes Association) guidelines on diagnosis of Diabetes Mellitus guidelines
Glycosylated HbA1c
- Method: HPLC(High Performance Liquid Chromatography)
- Specimen: EDTA WHOLE BLOOD
- Unit of measure: %
- Biological reference interval:
Non-Diabetic Level: < 5.7%
Pre Diabetic :5.7-6.4% %
Diabetic Level :>=6.5%
Goal :7.0%
Mean Blood Glucose Level
- Method: —
- Specimen: EDTA WHOLE BLOOD
- Unit of measure: mg/dL
- Biological reference interval: —
LIPID PROFILE
CHOLESTEROL
- Method: Cholesterol Oxidase,esterase,Peroxidase
- Specimen: SERUM
- Unit of measure: mg/dL
- Biological reference interval:
Desirable :<200
Borderline high :200-239
High :>240
HDL CHOLESTEROL
- Method: Direct
- Specimen: SERUM
- Unit of measure: mg/dl
- Biological reference interval:
Male: 40-60
Female: 50-60
NCEP ATP III guidelines
LDL CHOLESTEROL
- Method: Direct
- Specimen: SERUM
- Unit of measure: mg/dl
- Biological reference interval:
Optimal :<100
Near Optimal/above Optimal:100- 129
Borderline high :132-159
High :159-189
VeryHigh :>190
NCEP ATP III guidelines
TRIGLYCERIDES
- Method: Lipase/Glycerol Dehydrogenase
- Specimen: SERUM
- Unit of measure: mg/dl
- Biological reference interval:
Normal :<150 mg/dl
Borderline high:150-199 mg/dl
High :200-499 mg/dl
very high :>500 mg/dl
NCEP ATP III guidelines
VLDL CHOLESTEROL
- Method: Calculation
- Specimen: SERUM
- Unit of measure: mg/dl
- Biological reference interval: <30
Non-HDL Cholesterol
- Method: Calculation
- Specimen: SERUM
- Unit of measure: mg/dl
- Biological reference interval: <130
CHO / HDL RATIO
- Method: Calculation
- Specimen: SERUM
- Unit of measure: Ratio
- Biological reference interval:
Optimal<3.5
Goal <5.0
LDL/HDL RATIO
- Method: Calculation
- Specimen: SERUM
- Unit of measure: Ratio
- Biological reference interval: 1.5-3.5
NCEP ATP III guidelines
TGL/HDL Ratio
- Method: Calculation
- Specimen: SERUM
- Unit of measure: Ratio
- Biological reference interval:
Ideal :<2.0
High risk :>4.0
Very high risk:6.0
LIVER FUNCTION TEST(LFT)
BILIRUBIN TOTAL
- Method : Diazo
- Specimen: SERUM
- Unit of measure: mg/dL
- Biological reference interval: 0.1-1.2
BILIRUBIN DIRECT
- Method : Diazo
- Specimen: SERUM
- Unit of measure: mg/dL
- Biological reference interval: 0-0.3
BILIRUBIN INDIRECT
- Method : Diazo
- Specimen: SERUM
- Unit of measure: mg/dL
- Biological reference interval: 0.1 – 0.9
Aspartate aminotransferase(AST/SGOT)
- Method : IFCC
- Specimen: SERUM
- Unit of measure: U/L
- Biological reference interval:
Male: 5-35
Female: 5-30
Alanine aminotransferase(ALT/SGPT)
- Method : IFCC
- Specimen: SERUM
- Unit of measure: U/L
- Biological reference interval:
Male: 5-40
Female: 5-35
ALKALINE PHOSPHATASE
- Method : AMP
- Specimen: SERUM
- Unit of measure: U/L
- Biological reference interval:
Male: 53-128
Female: 42-98
GAMMA GT
- Method : Glutamyltransferase
- Specimen: SERUM
- Unit of measure: U/L
- Biological reference interval:
Male: 5-50
Female: 5-35
TOTAL PROTEIN
- Method : Biuret
- Specimen: SERUM
- Unit of measure: g/dl
- Biological reference interval: 6.0-8.0
ALBUMIN
- Method : Bromocresol Green(BCG)
- Specimen: SERUM
- Unit of measure: g/dl
- Biological reference interval: 3.5-5.2
GLOBULIN
- Method : Calculation
- Specimen: SERUM
- Unit of measure: g/dl
- Biological reference interval: 2.8-3.5
A/G RATIO
- Method : Calculation
- Specimen: SERUM
- Unit of measure: Ratio
- Biological reference interval: 1.1-2.5
AST/ALT
- Method : Calculation
- Specimen: SERUM
- Unit of measure: %
- Biological reference interval: 1-1.19
APOLIPOPROTEIN B
- Method : Nephelometry
- Specimen: SERUM
- Unit of measure: mg/dL
- Biological reference interval: <100
CRP-Highly Sensitive(hsCRP)
- Method : PEG Enhanced immunoturbidimetry
- Specimen: SERUM
- Unit of measure: mg/L
- Biological reference interval:
<1.00 — Low Risk
1-00 – 3.00 — Average Risk
>3.00 — High Risk
LIPOPROTEIN (A)
- Method : Nephelometry
- Specimen: SERUM
- Unit of measure: mg/dL
- Biological reference interval: <30
RENAL PROFILE(RFT or KFT)
UREA
- Method : Urease
- Specimen: SERUM
- Unit of measure: mg/dL
- Biological reference interval:
Male: 17-51
Female: 13-45
CREATININE
- Method : Creatinine amidohydrolase
- Specimen: SERUM
- Unit of measure: mg/dL
- Biological reference interval:
- Male: 0.5-1.3
- Female: 0.5-1.1
URIC ACID
- Method : Uricase
- Specimen: SERUM
- Unit of measure: mg/dL
- Biological reference interval:
Male: 3.6-7.5
Female: 2.5-6.7
Blood Urea Nitrogen(BUN)
- Method : Urease/GLDH
- Specimen: SERUM
- Unit of measure: mg/dL
- Biological reference interval: 6.0-20.0
CALCIUM
- Method : Arsenazo III
- Specimen: SERUM
- Unit of measure: mg/dL
- Biological reference interval: 8.4-10.4
PHOSPHORUS
- Method : —
- Specimen: SERUM
- Unit of measure: mg/dL
- Biological reference interval: 2.32-4.65
ELECTROLYTES
SODIUM
- Method : Direct ISE
- Specimen: SERUM
- Unit of measure: mmol/L
- Biological reference interval: 135-145
POTASSIUM
- Method : Direct ISE
- Specimen: SERUM
- Unit of measure: mmol/L
- Biological reference interval: 3.5-5.2
CHLORIDE
- Method : Direct ISE
- Specimen: SERUM
- Unit of measure: mmol/L
- Biological reference interval: 95.0 – 110
BICARBONATE
- Method : Calculated
- Specimen: SERUM
- Unit of measure: mEq/L
- Biological reference interval: 22-32
IRON STUDIES
Iron
- Method : Calculated
- Specimen: SERUM
- Unit of measure: ug/dl
- Biological reference interval: 41-141
Total Iron Binding Capacity (TIBC)
- Method : Saturation
- Specimen: SERUM
- Unit of measure: ug/dl
- Biological reference interval: 251-406
Transferrin Saturation
- Method : —
- Specimen: SERUM
- Unit of measure: %
- Biological reference interval: 20-50
ZINC
- Method : Nitro PAPS
- Specimen: SERUM
- Unit of measure: ug/dl
- Biological reference interval: 60-120
RBC MAGNESIUM
- Method : —
- Specimen: 3ml NA HEPARIN BLOOD
- Unit of measure: mg/dL
- Biological reference interval: 4.01-6.44
CYSTATIN-C
- Method : Nephelometry
- Specimen: 3ml NA HEPARIN BLOOD
- Unit of measure: mg/L
- Biological reference interval: 0.61-0.95
LIPASE
- Method : Turbidimetric U. V
- Specimen: SERUM
- Unit of measure: U/L
- Biological reference interval: 23-300
AMYLASE
- Method : Direct Substrate
- Specimen: SERUM
- Unit of measure: U/L
- Biological reference interval: 28 – 100
Creatine Phosphokinase(CPK)
- Method : IFCC
- Specimen: SERUM
- Unit of measure: U/L
- Biological reference interval:
- Male: 46-171
- Female: 34-145
lactate dehydrogenase (LDH)
- Method : Kinetic UV
- Specimen: SERUM
- Unit of measure: IU/L
- Biological reference interval: 125-246
MICROALBUMIN/CREATININE RATIO (ACR RATIO)
MICRO ALBUMIN-URINE
- Method : PEG Enhanced immunoturbidimetry
- Specimen: URINE
- Unit of measure: mg/dL
- Biological reference interval:
Normal/Non diabetic :<30
Microalbuminuria :30-300
Clinical albuminuria:>300
CREATININE-SPOT URINE
- Method : —
- Specimen: URINE
- Unit of measure: mg/dL
- Biological reference interval: 40-300
MICROALBUMIN/CREATININE RATIO
- Method : Immunoturbidimetry
- Specimen: URINE
- Unit of measure: mg/g
- Biological reference interval:
Normal : <30
Microalbuminuria: 30 – 299
Clinicalalbuminuria: > 300
SEROLOGY
FREE TESTOSTERONE
- Method : ELISA
- Specimen: SERUM
- Unit of measure: pg/ml
- Biological reference interval:
- Male: 1.0-28.28
- Female: 0.0-2.85
CLINICAL PATHOLOGY
URINE COMPLETE ANALYSIS (CUE)
COLOUR
- Method : Visual Inspection
- Specimen: URINE
- Unit of measure: —
- Biological reference interval: Pale Yellow
CLARITY
- Method : Visual Inspection
- Specimen: URINE
- Unit of measure: —
- Biological reference interval: Clear
PH
- Method : Dipstick,Double Indicator Principle
- Specimen: URINE
- Unit of measure: —
- Biological reference interval:
4.6 – 8.0
[4.6-6.9 Acidic
7.0 Neutral
7.1-9.0 Alkaline]
SPECIFIC GRAVITY
- Method : Dipstick,Polyelectrolyte/Bromothymol blue
- Specimen: URINE
- Unit of measure: —
- Biological reference interval: 1.005-1.030
GLUCOSE-URINE(F)
- Method : Dipstick,Glucose oxidase /Peroxidase
- Specimen: URINE
- Unit of measure: —
- Biological reference interval: Nil
PROTEIN
- Method : Dipstick,Protein error of indicators
- Specimen: URINE
- Unit of measure: —
- Biological reference interval: Negative
NITRITE
- Method : Dipstick,p-arsanilicacid/diazonium
- Specimen: URINE
- Unit of measure: —
- Biological reference interval: Negative
KETONE
- Method : Dipstick,Nitroprussiderection
- Specimen: URINE
- Unit of measure: —
- Biological reference interval: Negative
BILE PIGMENT&BILE SALT
- Method : Dipstick,Diazonium
- Specimen: URINE
- Unit of measure: —
- Biological reference interval: Absent
UROBILLINOGEN
- Method : Dipstick,modified Ehrlich aldehyde reaction
- Specimen: URINE
- Unit of measure: —
- Biological reference interval: Normal
BLOOD
- Method : Dipstick,Benzidine
- Specimen: URINE
- Unit of measure: —
- Biological reference interval: Negative
PUS CELLS
- Method : Microscopy
- Specimen: URINE
- Unit of measure: —
- Biological reference interval: 0-5/hpf
EPITHELIAL CELLS
- Method : Microscopy
- Specimen: URINE
- Unit of measure: —
- Biological reference interval: 0-5/hpf
RBC’S
- Method : Microscopy
- Specimen: URINE
- Unit of measure: —
- Biological reference interval: 0-2/hpf
CRYSTALS
- Method : Microscopy
- Specimen: URINE
- Unit of measure: —
- Biological reference interval: Nil
HYALINE CASTS
- Method : Microscopy
- Specimen: URINE
- Unit of measure: —
- Biological reference interval: 0-2 hyaline cast /lpf
PATHOLOGICAL CAST
- Method : Microscopy
- Specimen: URINE
- Unit of measure: —
- Biological reference interval: Nil
BACTERIA
- Method : Microscopy
- Specimen: URINE
- Unit of measure: —
- Biological reference interval: None
YEAST
- Method : Microscopy
- Specimen: URINE
- Unit of measure: —
- Biological reference interval: None
MUCUS
- Method : Microscopy
- Specimen: URINE
- Unit of measure: —
- Biological reference interval: Absent
IMMUNOLOGY
HOMOCYSTEINE
- Method : Enzymatic
- Specimen: EDTA PLASMA/SERUM
- Unit of measure: µmol/L
- Biological reference interval: < 15.0
THYROID PROFILE TEST – FREE
Free T3 (Triiodothyronine -Free T3)
- Method : CLIA
- Specimen: SERUM
- Unit of measure: pg/ml
- Biological reference interval: 2.6-5.2
Free T4 (Thyroxine Free)
- Method : CLIA
- Specimen: SERUM
- Unit of measure: ng/dl
- Biological reference interval: 0.77-1.99
TSH (Thyroid-stimulating hormone)-Ultra
- Method : CLIA
- Specimen: SERUM
- Unit of measure: uIU/ml
- Biological reference interval: 0.4-4.5
THYROID PEROXIDASE Ab (Anti TPO)
- Method : Chemiluminescence
- Specimen: SERUM
- Unit of measure: EU/ml
- Biological reference interval:
Positive : > 60
Negative : =< 60
THYROGLOBULIN Ab (Anti TG)
- Method : Chemiluminescence
- Specimen: SERUM
- Unit of measure: IU/ml
- Biological reference interval:
Negative :<4.5
Positive :>=4.5
INSULIN (F)
- Method : CLIA
- Specimen: SERUM
- Unit of measure: mU/L
- Biological reference interval: 2.6-24.9
BETA CELL FUNCTION
- Method : —
- Specimen: FLUORIDE PLASMA/SERUM
- Unit of measure: %
- Biological reference interval: —
VITAMIN B 12
- Method : CLIA
- Specimen: SERUM
- Unit of measure: pg/ml
- Biological reference interval: 120-911
VITAMIN D Total 25( OH)
- Method : CLIA
- Specimen: SERUM
- Unit of measure: ng/ml
- Biological reference interval:
Deficiency : < 20
Insufficiency : 21-29
Sufficiency/Optimal: 30–100
High : 51 – 100
Toxicity : > 100
IRON STUDIES
FERRITIN
- Method : —
- Specimen: SERUM
- Unit of measure: ng/ml
- Biological reference interval:
Male: 20-250
Female: 10-120
Immunoglobulin E (Total IgE)
- Method : Chemiluminescence
- Specimen: SERUM
- Unit of measure: IU/mL
- Biological reference interval: <160
PSA-Total (Prostate-specific antigen-Total)
- Method : CLIA
- Specimen: SERUM
- Unit of measure: ng/ml
- Biological reference interval: < = 2.0
TESTOSTERONE – TOTAL
- Method : CLIA
- Specimen: SERUM
- Unit of measure: ng/dl
- Biological reference interval: 270-1070
CORTISOL (AM)
- Method : CLIA
- Specimen: SERUM
- Unit of measure: µg/dl
- Biological reference interval: 7.5-23.56
ESTRADIOL(E2)
- Method : CLIA
- Specimen: SERUM
- Unit of measure: pg/ml
- Biological reference interval: 11.8-50
Follicle-stimulating hormone (FSH)
- Method : CLIA
- Specimen: SERUM
- Unit of measure: mIU/mL
- Biological reference interval: 1.8-15.4
PROLACTIN
- Method : CLIA
- Specimen: SERUM
- Unit of measure: ng/ml
- Biological reference interval: 2.1-17.7
ANTI MULLERIAN HORMONE(AMH)
- Method : CLIA
- Specimen: SERUM
- Unit of measure: ng/ml
- Biological reference interval: 1.14 – 9.17
IMMUNO CHEMISTRY
HELICOBACTER.PYLORI (H.PYLORI) IgG
- Method : ELISA
- Specimen: SERUM
- Unit of measure: Ratio
- Biological reference interval:
Negative: < 0.8
Indeterminate:0.8-0.9
Positive: >=0.9
Sex Hormone Binding Globulin (SHBG)
- Method : Chemiluminescence
- Specimen: SERUM
- Unit of measure: nmol/L
- Biological reference interval: 13.5-71.4